Copyright © 2023
Designed by Vex Design Studio
Animal studies are irreplaceable in investigating the relationship of dysfunction of specific brain microcircuits to behavioural manifestations of mental disorders. Animal models have suffered in the past from oversimplified extrapolation of observations in model systems to human-specific traits.
However, the emergence of tools for quantitative and automated measure of traits can overcome the influence of bias originating from experimentator or specific approaches to data collection and/or analysis. A number of devices measuring specific behvaioral deficits were employed by consortium members.
In the ‘In vivo’ part of the SAME-NeuroID platform we aimed to standardize workflows for automated measure of mice behavior. The focus was on monitoring social behavior as a neurobiological trait shared between many psychiatric disorders.
RESEARCH STRATEGY, the development of the in vivo component of the SAME-NeuroID platform has been performed in close collaboration between consortium partners. A real milestone was the workshop organized in Munich where members of two teams (Dr Schmidt and Dr Slezak) working on this topic met with colleagues from Paris Brain Institute (Dr. E. Bougiere) and EMC Rotterdam (Dr A. Badura). The brainstorm and knowledge exchange were instrumental for clarifying the scope of the planned tools and helped to arrange personal communication channels for frequent interactions between participants. Further, PORT researchers have accomplished several short stays at MPI and EMC to broaden the overview of techniques used in leading laboratories.
As a result, we successfully designed, constructed and developed at PORT a setup for videorecording of mouse cohorts along with a complete workflow for data analysis. Throughout the project, we worked closely with our partners. In particular, lively collaboration was launched between PORT and MPI resulting in parallel use of the protocols for inducing depressive-like behavior, parallel implementation of the workflow for automated analysis of social behavior, joint publications, and several common projects.
SOP 6. Chronic Social Defeat Stress-Paradigm in males and females.
SOP for chronic social defeat stress (CSDS), one of the most robust preclinical paradigms for eliciting depressive-like behavior in animals.
SOP 7. Automated Analysis of Mice Social Interactions
The SOP is a result of collaborative work of SAME-NeuroID partners which led to a coherent workflow of social behavior analysis.
SOP 8. Behavioral assessment of CSDS effects on Social Interaction
The SOP for complementary analysis of alterations of social behavior induced by chronic stress covering both, classical approach (3-chamber social interaction test) and unbiased measure of behavior
With a relentless pursuit of knowledge, we unravel the complexities of the mind, unraveling the mysteries that hinder mental well-being. Join us as we pave the way for breakthroughs in neuroscience and offer hope for a brighter future
With unwavering determination, we delve into the depths of the human mind, pioneering breakthroughs that inspire a brighter future for mental well-being and transform lives.
Copyright © 2023
Designed by Vex Design Studio