Copyright © 2023
Designed by Vex Design Studio
Scientific aim of the project is to establish the platform of Standardized Approaches to Modelling and Examination of Neuropsychiatric Disorders (SAME-NeuroID) at Łukasiewicz – PORT.
The components of the SAME-NeuroID platform are two types of complementary assays covering key aspects of target validation: in vivo assays for evaluating social behaviour deficits in rodents, and in vitro assays for examining cellular phenotypes related to psychiatric diseases in the context of human neural cells.
In the ‘In vitro’ part of the SAME-NeuroID platform we aimed to implement and standardize workflows for the differentiation of human induced pluripotent stem cells (iPSCs) into neural lineages, as key tools for the modeling and study of neuropsychiatric disorders.
We successfully implemented and adapted at Łukasiewicz – PORT five key protocols that are highly valuable for building an iPSC-based in vitro platform that may be the basis for drug discovery or disease mechanism study with high commercialization potential.
These protocols include iPSC differentiation into astrocytes, neuronal networks, and adherent cortical organoids. In each procedure, a starting point is the existing hIPSC line with intermediate steps include the generation of embryoid bodies and Neural Progenitor Cells (NPCs).
SOP 1. Generation of Embryoid Bodies (EBs) and Neural Progenitor Cells (NPCs) from iPSCs
The SOP describe first steps of differentiating hIPSC towards neural lineage.
SOP 2. FACS-based selection and freezing of NPCs
The SOPs of obtaining pure population of NPCs from EBs, thanks to the specific set of cell surface signatures and Fluorescence Activated Cell Sorting (FACS).
SOP 3. Differentiation of NPCs towards neural networks
The SOPs summarizes robust method of differentiating NPCs into functional neurons.
SOP 4. Differentiation of NPCs towards astrocytes
The SOPs of differentiating NPCs to pure population of astrocytes by implementing leukemia inhibitory factor (LIF) and bone morphogenetic protein 4 (BMP4), as differentiation factors.
SOP 5. Differentiation of NPCs towards cortical organoids (384-well plate format)
The SOPS for robust generation of highly similar brain organoids – three-dimensional in vitro cellular structures that recapitulate the complexity of the neural network’s formation and functioning.
With a relentless pursuit of knowledge, we unravel the complexities of the mind, unraveling the mysteries that hinder mental well-being. Join us as we pave the way for breakthroughs in neuroscience and offer hope for a brighter future
With unwavering determination, we delve into the depths of the human mind, pioneering breakthroughs that inspire a brighter future for mental well-being and transform lives.
Copyright © 2023
Designed by Vex Design Studio