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SHORT TERM VISIT
Young scientists from Łukasiewicz-PORT have beem on a two-week (26.05-08.06.2024) lab visit to one of the SAME-NeuroID partner institutions, the Paris Brain Institute (ICM). The primary purpose of the workshop, organized by the Stem Cells Core Facility, was to gain practical skills and theoretical knowledge about the reprogramming of human somatic cells, such as fibroblasts and peripheral blood mononuclear cells (PBMCs), into induced pluripotent stem cells (iPSCs). Induced pluripotent stem cells possess an almost unlimited capacity for cell division, are immunologically neutral, and can differentiate into nearly any tissue type. Therefore, the generation of iPSC is a powerful tool that can be utilized in personalized therapies, drug screening approaches, and in vitro disease modeling.
Ewa Mrówczyńska, PhD (Post-doc, RG Mechanisms of Neurodegeneration) and Joanna Sadlak-Buda, MSc (PhD Student, RG Synaptogenesis) acquired substantial theoretical and practical expertise in the methods of reprogramming somatic cells to iPSCs using Sendai virus in culture under non-feeder conditions. The intensive course included step-by-step guidance from the preparation of somatic cells to their full transformation into stable and pure iPSCs, and a range of techniques for molecularly and functionally characterizing newly generated iPSC lines.
SHORT TERM VISIT
Observation and active participation in each crucial step of the protocol have allowed them to learn how to independently and efficiently perform such cell reprogramming at Łukasiewicz-PORT.
All these positively contributed to the personal development of young scientists but also enhanced the research capabilities at Łukasiewicz-PORT by empowering the implementation of advanced methodologies for studying neuropsychiatric disorders using patient-derived cells.
In addition, young scientists from Brain Development RG, who utilize iPSC technology, provided a brief introduction to their research and methods, particularly focusing on cortical organoid formation. This exchange of knowledge and techniques has significantly enriched the skillset of Łukasiewicz-PORT researchers, allowed them to establish valuable professional connections with scientists from SAME-NeuroID partner institutions, and opened new avenues and inspiration for future collaborative projects.
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Copyright © 2023
Designed by Vex Design Studio